Zebrafish was chosen as a model organism to explore the neurotoxic effects of combined exposure to three microplastics (PP, PE, PVC) and triclosan (TCS). The results showed that microplastics (MPs) could be used as the carriers of TCS in vivo, and the combined toxicity of MPs and TCS was mainly synergistic. Compared with TCS, SOD in TCS+PP, TCS+PE and TCS+PVC groups decreased by 10.3%, 12.4% and 7.9%, and AchE activity decreased by 10.8%, 1.5% and 6.1%, respectively. The 5-HT content was increased by 83.2%, 37.7% and 59.3% (p<0.05), respectively. Compared with TCS+PE, the 5-HT content in TCS+PP group was increased by 33.1%, showing a significant difference (p<0.05). Combined exposure of PP-MPS and TCS produced stronger neurotoxicity. Metabolome analysis showed that the TCS+PP complex group induced 98 metabolites with specific changes, and these metabolites produced more serious neurotoxicity to the brain by affecting amino acid metabolism, glycerophospholipid metabolism, sphingolipid metabolism, arachidonic acid metabolism and neuroactive ligand receptor interaction and other metabolic processes.