Carbon capture, utilization and storage (CCUS) are the key technologies to address global warming. CCUS technologies reduce the CO_2 content in the atmosphere by CO_2 capture and storage. Replacing the use of fossil fuels with CO_2 could further reduce the net emission of CO_2. The applications of CCUS technologies strongly rely on the comprehensive studies of the physical and chemical processes involving CO_2 at micro scale. Microfluidics provides a platform to manipulate fluids at micro or nano scale and reveals the laws of fluid motion. Microfluidics plays an important role in the whole process of CCUS. This review summarizes the advantages of microfluidic technology, including precise manipulation of fluid, large specific surface area and enhanced heat and mass transfer. The applications of microfluidics in CCUS are introduced in detail. Applications of microfluidics for CO_2 capture include the rapid screening of CO_2 adsorbents, formulation optimization and material preparation, while those for CO_2 utilization mainly consist of electrocatalytic CO2 reduction reaction (CO_2RR), photocatalytic CO2RR, catalyst preparation and photosynthesis. The microfluidics for CO_2 geosequestration is mainly associated with fluid dynamic analysis and geological model construction. Finally, the challenge and opportunity of microfluidics in the field of CCUS are summarized and outlooked.